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Biology, 01.12.2021 04:10 jenny3661

g You want to study a eukaryotic protein in a cell-free reaction system (just protein in buffer), so you first need to purify your protein. You clone the gene and its promoter region, then incorporate it into a plasmid. After transforming the plasmid into bacterial cells, you note that general protein expression has initiated but your protein is not observed after homogenizing the cells and analyzing the lysate. Why might this be true and how would you resolve it

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