To estimate the number of cleavage sites in a particular piece of DNA with a known size, you can apply the formula, N/4n where N is the number of base pairs in the target DNA and n is the number of bases in the recognition sequence of the restriction enzyme. If the recognition sequence for HindIII is AAGCTT and the Escherichia coli genome contains approximately 4,640,000 bp, how many cleavage sites would you expect?
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To estimate the number of cleavage sites in a particular piece of DNA with a known size, you can app...
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