You want to insert an ORF into pT5 plasmid. The DNA fragment is an open reading frame with ATG on the 5’end. pT5 plasmid contains a bacterial promoter that spans from 0.5 to 0.8 kb and goes clockwise, an EcoRI site at 0/6 kb, a HindIII site at 1 kb, a BamHI site at 3 kb. Your ORF is a HindIII fragment (2 kb long) with an EcoRI site at 1 kb and a BamHI site at 1.5 kb. Required:
a. Draw the recombinant plasmid to show the right orientation of the ORF in the plasmid.
b. Which enzyme can you use to find out the orientation of the ORF after ligation?

You decide that the introduction should also discuss the extremophiles that are referred to as the archaea. these single-cell organisms are considered "extremophiles" due to their ability to survive and reproduce in environmental conditions that would be hostile for most living organisms. archaea species have been isolated from highly acidic sulfur springs, ocean floor thermal vents with temperatures that exceed boiling, and subarctic ice well below freezing. while still considered to be prokaryotic, the archaea have numerous differences that place them apart from the bacteria. choose the characteristics that separate the archaea from other prokaryotic cells. select all that apply. view available hint(s) select all that apply. archaea lack true peptidoglycan in their cell walls. the morphology of the cell is rigid and is geometric in shape, similar to a sphere or cylinder. the cytoplasmic membrane lipids of archaea have branched or ringform hydrocarbon chains. all currently identified and characterized archaea have been linked as the causative agent to an animal or human disease.

What did not occur in the proterozoic eon a)the oxygen levels increase greatly in the atmosphere,b)the emergence of the eukarya , c)muti cella’s eukaryotic organisms began to diversify,d)anaerobic bacteria kept thriving with more oxygen

What are examples of the plant life and animal life that can be found in each type of terrarium