A researcher studying a line of cancer cells notices that cellular content of RhoBTB3 and Cyclin E1 increase and decrease through the cell cycle.

a. He hypothesizes that RhoBTB3 signaling regulates the production of Cyclin E1 and the entry of the

cell into mitosis. Based on the data below, explain why his results do not support this hypothesis.

Identify what conclusions are supported by this data. -

b. Suppose that the researcher can engineer a cell line in which RhoBTB3 expression can be induced

by the addition of a drug. Design an experiment using this cell line to test whether RhoBTB3

regulates the production of Cyclin E1. Describe what results he can expect if his hypothesis is

correct.