You have a stock of BSA protein at the concentration of 100pg/mba You want to do a Bradford assay to make a standard curve and determine the protein concentration of
unknown samples. To make your standard curve, you should make the following
dilutions of the BSA to use as your standards. Note that final volume of standards
changes for each.
Show your work below for the amounts of BSA stock and water to mix to make
each of the following standards:
Standard #1: 1000L of 50ug/mL BSA
Volume of BSA stock:
Volume of water:
Standard #2: 100L of 25pg/mL BSA
Volume of BSA stock:
Volume of water: