You are performing a biochemical purification of enzymes involved in DNA replication. You have purified the replisome. You wish to purify the primosome. You perform further separation techniques on your purified replisome. How will you test to determine that you have purified the primosome

Confirm that the sample has both helicase and primase activities, but not the ability to synthesize DNA

            1. Helicase enzymes that unwind and separate the DNA strand

            2. Replication factor C (RFC), a DNA-dependent ATP that acts as an activator of DNA polymerase.

           3. Proliferating cell nuclear antigen (PCNA)

           4. DNA gyrase/topoisomerase that cuts and reseals DNA strands, which is essential for DNA synthesis.

           5. Primase enzyme that synthesizes short RNA fragments called primers.

          6. DNA polymerase III, an enzyme that catalyzes the polymerization of deoxyribonucleotides into a DNA strand.

           7. DNA ligases enzymes that form phosphodiester bonds at a single-strand break in DNA

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answer; i do believe that the correct answer is; ///mendelian genes are at specific loci on the chromosome and, in turn, segregate during meiosis;

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