The researcher places the lacZ gene in the cloning region so that it is disrupted by DNA inserts. Place the remaining steps for this process in the correct order. Not all steps will be used. a. Blue colonies that survive On the culture medium contain the recombinant plasmid.
b. Grow cells containing the recombinant plasmid in a medium with ampicillin and X-gal (a galactose derivative).
c. All the fragments have the sticky ends 5 '—GCGGCCGC—3'.
d. All the fragments have identical sticky ends: 5' —GGCC—3'.
e. Use DNA ligase to join the sticky ends of the DNA sample and the plasmid.
f. White colonies that survive on the culture medium contain the recombinant plasmid.
g. Remove colonies containing the recombinant plasmid from the culture medium and continue growing.

It's speed of rotation.

Proteins are the workhorses of the cell, controlling virtually every reaction within as well as providing structure and serving as signals to other cells. proteins are long chains of  amino acids  , and the exact sequence of the amino acids determines the final structure and function of the protein. instructions for that sequence are encoded in  genes  . to make a particular protein, a messenger ribonucleic acid (mrna) copy is made from the gene (in the process called  transcription  ), and the mrna is transported to the  ribosome  . protein synthesis, also called  translation  , begins when the two ribosomal subunits link onto the mrna. this step, called initiation, is followed by elongation, in which successive amino acids are added to the growing chain, brought in by transfer rnas (trnas). in this step, the ribosome reads the  nucleotides  of mrna three by three, in units called  codons  , and matches each to three nucleotides on the trna, called the anticodon. finally, during termination, the ribosome unbinds from the mrna, and the  amino acid  chain goes on to be processed and folded to make the final, functional protein

It depends on the surface of the levels. the rougher the surface, the greater is the frictional force. for rougher surfaces, you would need to exert greater force to move the object. the concept is that, you must overcome the frictional force in order to bring the object into motion. this is the reason why we add lubricants to the joints so they could easily move. this answer was on

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