The expression and purification of rGFP started with the growth of the bacterial culture and ended with the elution of rGFP off the Ni 2-agarose column. Assuming the column was not overloaded, which of the following changes to the procedure would result in an increase in the yield of rGFP in the elution fractions?
A. Use a 1ml bed volume instead of a 0.5ml bed volume.
B. Induce the culture longer than 3 hours before harvesting.
C. Increase the number of washes before switching to elution buffer.
D. Use a 20ml pellet instead of a 15 ml pellet.
Answers: 1
Biology, 21.06.2019 13:10
*will mark brainliest* certain species of are now recognized as indicators of environmental pollution. a. lichen b. mushroom c. yeast d. none of the above
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Biology, 22.06.2019 00:50
What would be the result if crossing over did not happen during meiosis in humans? a. there would be less genetic variation in humans. b. parents would be more likely to look like their children. c. the human population could not reproduce. d. children would have more chromosomes.
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Biology, 22.06.2019 06:00
Monomers are the building blocks of larger molecules, called polymers. for example, proteins are composed of chains of amino acids that are linked together. cellulose is a polymer that makes up plant cell walls. cellulose is made from a chain of c6h10o5 molecules. which monomers are most likely used to produce cellulose? why?
Answers: 3
The expression and purification of rGFP started with the growth of the bacterial culture and ended w...
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