In a separate maddyase experiment using (Et)-10nM, the reaction velocity is measured as 3uMS. What is the substrate concentration used in the experiment

hello your question has some missing data attached below is the missing data

Applying the Michaelis-Menten Equation

An enzyme is found that catalyzes the reaction X ⇋ Y Researchers find that kcat for this enzyme is 600s^-1 and carry out several additional experiments.

answer :

substrate concentration [ x ] = 10⁻⁵ M

Explanation:

Given data:

[Et ] = 10 * 10⁻⁹ M ,  

Reaction velocity ( V₀ ) = 3* 10⁻⁶ Ms⁻¹

applying Michaelis-Menten  Equation

= 3* 10⁻⁶ =  ,    

= ( 3 * 10⁻⁶ )( x ) + ( 3 * 10⁻¹¹ ) = ( 6 * 10⁻⁶ ) ( x )

hence the substrate concentration [ x ] = 10⁻⁵ M

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