A fellow student attempts to follow the protocol. They know that the GCE that was applied to the column was fluorescing, however fractions E1-E10 do not fluoresce. What two student errors could have occurred during the Ni 2 Agarose purification procedure to obtain this result?

when you breathe in, or inhale, your diaphragm contracts (tightens) and moves downward. this increases the space in your chest cavity, into which your lungs expand. the intercostal muscles between your ribs also enlarge the chest cavity. they contract to pull your rib cage both upward and outward when you inhale.

d. groundwater flows through the subsoil and deposits minerals.