Quizlet: Anion Exchange Chromatography I. Cation Exchange Chromatography C. Hydrophobic Interaction Chromatography J. Size exclusion chromatography (also known as gel filtration chromatography). - Salting out H. SDS-PAGE - Western Blot - Affinity chromatography - Isoelectric focusing - 2-D Gel Electrophoresis A. Smallest proteins move faster that smaller proteins. B. Uses immobilized small biologically relevant molecules or metal ions as the stationary phase. C. Protein is eluted by lowering the salt concentration. D. Requires a pH gradient. E. Requires that protein is transfered from a gel to a membrane. F. Often performed at a pH