Imagine scientists have discovered a new antibiotic that inhibits transcription. To determine which subunit of RNA polymerase holoenzyme (alpha, beta, beta-prime, omega, or sigma) the antibiotic targets, researchers took the approach used to determine that rifamycin targets the beta subunit of RNA polymerase. RNA polymerase subunits were isolated from antibiotic-resistant (R) and antibiotic-sensitive (S) strains of bacteria, mixed in vitro, and tested for transcriptional ability. The results are presented below. Based on the data, which subunit is the target of the antibiotic RNA polymerase subunit combinations that transcribed in the presence of the antibiotic 1. R-alpha, beta-prime, and sigma mixed with S-alpha, beta, and omega 2.R-beta, beta-prime and sigma mixed with S - alpha and omega 3. R-beta-prime, omega, and sigma mixed with 5 - alpha and beta RNA polymerase subunit combinations that did not transcribe in the presence of the antibiotic 1. R-alpha and omega mixed with S - alpha, beta, beta-prime, and sigma 2.R-beta, omega, and sigma mixed with S - alpha and beta-prime 3. R-alpha and sigma mixed with S-beta, beta-prime and omega Choose one: A. beta B. beta-prime C. sigma D. omega E. alpha