You are trying to purify a protein that is soluble in a solution of 2 M ammonium sulfate. After centrifugation to remove other proteins that have precipitated at this high salt concentration, you recover the supernatant to assay the target protein's activity in a cell culture system.
(a) Explain why the cell dies when incubated with the supernatant.
(b) What procedure could you preform to correct the problem?
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You are trying to purify a protein that is soluble in a solution of 2 M ammonium sulfate. After cent...
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