What do we now have to do to successfully use a cloned gene to transform mice?

To transform the mice, it can be done by genetic alteration and engineering which is the use of DNA insertion process either with the use of the current CRISPR technology or needle insertion. The use of electroporation. And lastly, inject a strong virus to the mice.   

brain development. a child's brain undergoes an amazing period of development from birth to three—producing more than a million neural connections each second. the development of the brain is influenced by many factors, including a child's relationships, experiences and environment.

explanation: