On a pedigree, what would you do with an individual that didn't say if they have the trait or not. would you still include it in the pedigree and if you would, would you do something to it?

If they are related to the people that have said trait you would use a punnet square to find whether they have the trait if necessary, if you don't need to know if they have the trait then you don't really need to add them, but it is better to have them in the pedigree for a more precise answer, at least that's what i think.

Rna polymeras should attache to the promoter.