Please help me and no file
The question is in the picture and this is biology

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.

if you add everything up correctly you should get 69 i got this because 50 plus 19 is it

the answer is true when observing or determining population size, a direct observation is use, in which each organism is counted in a population or in an area. one benefit of this measure is that you get the exact population count but it will take a long time to  determine  the  size  of a  population.