You are interested in whether the protein you are studying for your dissertation, ZyxR, is capable of binding to and regulating expression of the ZyxABC operon. ZyxR is a 24kDa protein with a slightly negative overall charge and no known inherent tags. ZyxR is known to bind to the zyxY gene to regulate expression. yopA (100bp) is not regulated by ZyxR. The first step in this process is to determine whether ZyxR is capable of binding to the regulatory regions surrounding the zyxABC operon. Give one potential strategy that you could use to purify ZyxR. Your strategy should be a multistep process beginning with cell lysate (containing millions of proteins) that you will take to purify the protein, should address potential shortcomings of each step, and should attempt to rectify those shortcomings. If your strategy requires any special conditions or changes to ZyxR, you should include those in the details. Drawings of your strategy/steps are highly encouraged!

Part e - sequence of metabolic processes each of the different cellular metabolic pathways occurs in a specific order. consider aerobic cellular metabolism from the beginning to end. what is the sequential order of the metabolic processes that starts with glucose and results in the production of carbon dioxide, water, and atp? put the following in the correct sequential order. rank from earliest to latest. to rank items as equivalent, overlap them. view available hint(s) acetyl coaglycolysiscitric acid cycle pyruvate finishstartstart background image electron transport chain background image finish background image submit cellular metabolism is often expressed as an equation. this equation puts into perspective, on a general scale, the substances needed to start cellular metabolism and the products that come out of it. the equation represents the overall product yield after every step of cellular metabolism is complete (i.e., glycolysis through the electron transport system). in this part of the tutorial you task will be to put together the equation that represents cellular metabolism. you will also learn the products that result from each metabolic pathway and the role that some these products play in the production of atp.

Which of these is not a nucleotide base found in dna?

Ascientist is explaining how background cosmic radiation gives evidence of the big bang theory. she says that radiation has been detected everywhere equally as heat in the universe and is increasing. this has been shown to be residual radiation from the big bang. what error did the scientist make?