You have been hired by a new biotechnology start up and placed in charge of cloning their new "miracle" gene. Describe the steps (in order) you would follow to produce
some transgenic bacteria (GMO) so the company can start manufacturing their new
product and get it on the market. What vector would you use and what enzymes?
How would you identify and isolate the transformed cells?

Astudy by solloch and et. al., in 2017, gives the map above which shows the frequency of alleles with a ccr5-delta32 mutations over 87 different countries. this mutation deletes the presence of a co-receptor (ccr5) on the outside of human t-cells (lymphocytes). some viruses, such as the one responsible for the black death and human immunodeficiency virus (hiv), require this receptor for attachment to host cells during the infection process. the black death was an epidemic that passed over northern europe during the 14th century killing nearly 60% of europeans. according to this information, which explanation best explains why northern europeans show a greater immunity for hiv than some other parts of the world?