First, Choose... stain is applied, a series of rinses are used, and then a Choose... stain is applied. A Choose... bacteria culture will stain purple or blue, while a Choose... bacteria culture will stain pink.

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primary; counter; Gram-positive; Gram-negative

Explanation:

The illustration is about the Gram-staining procedure.

The procedure is used to distinguish between the bacteria that have a thick cell wall capable of retaining the color of a dye (Gram-positive) or otherwise (Gram-negative).

A smear of the bacterium to be tested is heat-fixed to a clean glass slide after which a suitable primary stain is added, allowed to stay for a few seconds, and rinsed. A mordant is then added, rinsed before a counter-stain is added, and also rinsed. The slide is then allowed to drain off water and then viewed under the microscope using the oil immersion. Gram-positive bacteria will appear blue or purple, taking up the color of the stain while Gram-negative bacteria will appear red under the microscope.

crystal violet, safranine, gram-positive, gram-negative

Explanation:

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