The researchers introduced a small RNA complementary to a portion of the mRNA encoding protein A into the Brec-MUT cells growing in a petri dish. The researchers claim that the treated cells will fail to stimulate blood vessel formation. Based on information provided, provide reasoning to support their claim.

Delivered small RNAs can inhibit protein A production through the RNA interference (RNAi) mechanism, and thus impairs angiogenesis  

Explanation:

The pregnancy-associated plasma protein-A is a protease enzyme involved in the formation of new blood vessels by increasing insulin-like growth factor I (IGF-I) bioavailability. Moreover, small RNAs (<200 nucleotides in length, generally 18 to 30 nucleotides) are non-coding RNA molecules that function in RNA silencing through the RNA interference (RNAi) pathway. Small RNAs are widely used in molecular biology laboratories because they can be delivered into specific cells in order to silence target mRNAs such as, in this case, the mRNA encoding protein A, by complementary base pairing and thereby inducing translational repression. In consequence, mRNAs complementary to delivered small RNAs are silenced through RNAi pathways, i.e., by cleavage of the target mRNA and/or mRNA destabilization.

tsd is temerature dependent sex determination.

tsd: it is a type of environmental sex determination in which the temperature experienced during embryonic/ larval development (ambient temperature for development) determine the sex of the offspring.

crocodile displays the tsd where the ambient temperature of the developing egg determines the individual's sex. for example in the american alligator's eggs, incubation at 33 ºc produces mostly males, while incubation at 30 ºc produces mostly females. so if the american alligator's egg will get the ambient temperature (33 degree celcius)during embryonic development from egg, the offspring will be male and same for the female sex determination.