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Biology, 04.03.2021 04:50 tomasholguin

Observe the plates under the results section for the pour plate method for reference. While preparing your dilutions for the pour plate method, you work a little too slowly on your third tube [1:100 dilution] and you tube of agar solidifies after you inoculate the tube with your microbe, but before you can pour the plate. You and your partner decide to reheat the tube, melt the agar, and pour the plate. Once the plate solidifies, you incubate the plate at 37 degrees Celsius along with your other plates. You return to class 48 hours later and retrieve your plates from the incubator. Your first two plates with your 1:1 dilution and 1:10 dilution are covered in colonies similar to the plates in the result section. However, your third plate [1:100 dilution] has absolutely no growth on the plate. What happened?

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