The proteome solution was fractionated by ion-exchange chromatography. What type of ion-exchange resin will bind RNAP at pH 7.4

Explanation:

There is a lot of missing information in the question but I will help you with the explanation that can help you understand the question and at the end provide a solution to it correctly.

Ion exchange chromatography is necessary and is used for the separation of charged molecules. In this method or process, ionic solutes display reversible electrostatic interactions accompanied by the charged stationary phase, this usually comprises an insoluble matrix together with a covalently attached ion (ion exchanger). The solutes which are introduced in the column, perhaps(- ve) charged, (+ ve) charged, or neutral under a given condition.

So, the higher the charge density, the stronger the interaction.

pI of RNAP= 5.34

The pH of the experimental condition = 7.4

So, if pH is > pI of a solute, the solute gives H+ ion and converts to be (- ve) charged.

Thus, in this scenario (- ve) charged RNAP attaches to the (+ ve) charged ion exchanger. So, an anion exchanger (which is a basic ion exchanger) is imbibed in the separation of the anion in ion exchanger chromatography.

sample of such anion exchanger is DEAE - cellulose (Diethylaminomethyl).

answer: decrease

a limiting factor is the one which decrease the abundance, distribution and causes extinction of the population of species in a ecosystem. here, predators including hawks, owls and snakes are the limiting factor for the population of mexican free tailed bat.   although bats are nocturnal animals as they forge for food at night these predators also may remain active during night which can decrease the population of the mexican free-tailed bats. also, these bats eat only one type of insects that are moths. the food can also be a limiting factor as all 400 members are dependent upon same type of food needs that are moths. the decrease in the moth population can cause decrease in population size of the mexican free-tailed bats.

therefore, the scenarios will most likely to decrease the population size of the mexican free-tailed bats.