How long was each incubation period for the evolution experiment

Each culture grew to ≈1–2 × 1010 cells, which were pelleted by centrifugation, spread on an MC plate, and incubated for 45 days.

Interacting group of various species in a common location. for example, a forest of trees and undergrowth plants, inhabited by animals and rooted in soil containing bacteria and fungi, constitutes a biological community.

The answer to this will be from your lab notes/procedure. it would be best to ask another student who participated in the lab as we do not know what was done, what enzyme or if the temperature is celsius or fahrenheit. if i were to guess though i'd say the rate stopped due to enzyme denturing at high temperature