Put the following 6 steps in order to indicate the overall experimental flow. a. Quantify extracted protein
b. Transform BL21(DE3) w/ pDHFR
c. Run purified protein on gel to verify by SDS-PAGE and Western Blot
d. Generate pDHFR expression vector
e. Induce DHFR protein synthesis in E. coli with IPTG
f. Purify DHRF protein by affinity chromatography (IMAC)

relative dating involves things like higher layers are most recent than rocks lower in the sequence. this can be extended to the known sequence of fossils. rocks containing rabbit fossils are later than those containing dinosaur fossils.  

absolute dating methods mainly use radioactive elements that have known rates of decay and produce recognizable products. for example potassium 40 decays to produce argon. once a rock has solidified the argon is trapped. by measuring the argon and potassium0 you can get an accurate number for the age.  

some other methods are good for archaeological ages. dendrochronology involves recognizing the sequence of tree rings. the orientation of magnetized particles in baked clay can be used to date a fire.

hope this .. .

this protocol provides a detailed procedure for agarose-based ip to purify and enrich the protein of interest.

answers are:

lysogenic     viral dna merges with host cell dna

lysogenic     no symptoms

lytic               viral dna takes over

lysogenic     virus does not destroy host cell

lytic               virus destroys host cell

lytic               induces symptoms of viral infection

lysogenic       cell reproduces and spreads provirus