What procedures did caravaggio have to follow in order to take advantage of bioluminescence in his chiaroscuro style of painting in his tume

He used fireflies

Explanation:

there are so many examples for that in different areas, like biology experiment carried out in our lab recently.

here's one link:

the solute can move "downhill," from regions of higher to lower concentration, relying on the specificity of the protein carrier to pass through the membrane. this process is called passive transport or facilitated diffusion, and does not require energy.