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You find a solid tumor that contains a loss of function mutation in a tumor suppressor gene. You send the samples to a lab for analysis. The lab proposes to use a plasmid vector to add back a wild type copy of this gene in the tumor. Would this scheme work to limit the growth of the tumor cells? Assume that it is possible to deliver the plasmid vector to all tumor cells.
a. No, gene therapy is never used for treatment of human diseases.
b. No, it's a loss of function mutation, so more gene product is not needed.
c. Yes, it would provide a wild type copy of a tumor suppressor gene.
d. Yes, the plasmid would stimulate growth factor production and upregulate cell cycle.
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