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Biology, 05.12.2020 03:20 c1100321311

We can use the Vm to determine how many Units of Catalase there are in our sample Determine how many Units of Enzyme there were per gram of your piece of liver (U/g) Assume that all the Catalase was extracted from your piece of Liver into the 30 mL of buffer. Note that l Unit (U) is equal to the amount ofenzyme able to convert 1 μmole of H2O2 to products per minute in the reaction. Use the Vmax that you calculated in your Non-Linear Regression plot to help you do this. Hint: Convert our VaS(umoles H,0, consumed per second) to umoles min. Consider the volumes that we used and that we diluted our enzyme solution. Figure out how many Units of Enzyme were applied to the filter disk, then from the 5μ1 work backwards considering all dilutions.)

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