Parthenogenesis is a method of reproduction in which
a. an edd develops after being fertilized by a sperm
b. the parent animal divides into two new animals
c. the offspring develops as an outgrowth of the parents body wall.
d. an egg develops without being fertilized by a sperm

creative diagnostics provides a protocol for total protein extraction based on ripa which is widely used.

tsd is temerature dependent sex determination.

tsd: it is a type of environmental sex determination in which the temperature experienced during embryonic/ larval development (ambient temperature for development) determine the sex of the offspring.

crocodile displays the tsd where the ambient temperature of the developing egg determines the individual's sex. for example in the american alligator's eggs, incubation at 33 ºc produces mostly males, while incubation at 30 ºc produces mostly females. so if the american alligator's egg will get the ambient temperature (33 degree celcius)during embryonic development from egg, the offspring will be male and same for the female sex determination.