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Biology, 02.12.2020 16:50 anonymous1813

Most of the techniques, such as blotting, cloning, PCR, etc., are dependent on intermolecular attractions (annealing) between different populations of nucleic acids. Length of the strands, temperature, and percentage of GC nucleotides weigh considerably on intermolecular associations. Why should multiplex PCR be any different than single primer set PCR in terms of dependability and ease of optimization

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