A lab assistant needs to mix up the following reaction to prepare DNA for an experiment:. 20ul of a DNA sample
20ul of enzyme
40 ul of reaction buffer
120ul of water
The smallest size tube that will hold the entire reaction is:. [0.025, 0.25, or 2.5] ml:
Which of the following pipetting devices could be used to accurately measure all the needed volumes? [P20, P200, P1000 or 1 ml graduated pipet with electronic pipet-aid]

Explanation:

The total solution formed after mixing the components will be

20 μl + 20 μl + 40 μl + 120 μl = 200 μl

The smallest size tube that will hold the entire reaction is:

Firstly convert 200μl to ml

1000 μl = 1 ml

200 μl = X

X = 200 x 1/1000

X = 0.2 ml

The smallest size tube (from the options provided in the question) is 0.25 ml.

The pipetting device that could be used to accurately measure all the needed volumes must be able to measure 20 μl, 40 μl and 120 μl.

The best pipette to accurately take these measurements individually is the P200 micropipette because it has an accurate/useful range of between 20 μl - 200 μl

from almost the creation of the first true maps of the earth, people started seeing how continents would be able to fit together.   in particular, people noticed that south america fits almost exactly into africa.

you should be aware that while world maps were around early in the 1600’s and better defined by the end of the 1700’s, those maps were not ‘public’ but were treated as state secrets.   and so it was not till much later that the broader ‘science’ community had access to good quality and accurate world maps.

get the whole story here…

it is now known that most of the major continental masses can be fit together in a jigsaw process.   in fact we now know that the continents were indeed once all joined together as one land mass – the supercontinent of pangaea.

if you look at most world maps you will ponder how this is evidence, as the continents really don’t appear to ‘fit’ together very well.   what you need to understand is that the vast majority of maps are drawn using a map projection that has the north and south poles (which are points) are a line the width of the map at the top and bottom.   this means that the map distorts the true shape of the earth’s land and oceans as you love closer to the poles.     some other map projections distort less, but have the map shape looking like segments of an orange.   see here for a range of map projections.   check out the way greenland changes size in each!

what is more remarkable, is the next piece of evidence when added to the shape of continents.

location of mountains and fossils

what is remarkable, when you reassemble all the continents together, the ancient mountain ranges (many now eroded to their cores) and the location of rock types and fossils all match up.