Which of the following does NOT effect the rate of enzyme reaction? Question 1 options: pH Temperature Amount of product Substrate Concentration Using the data collected during 2.16 Lab Enzymes, what was the optimum pH for the enzyme amylase? Question 2 options: 9 5 3 7 Based on the data from Lab 2.16 Lab Enzymes, how did the amount of substrate affect the reaction rate. Question 3 options: More substrate = more product. More substrate = less product. Less substrate = average product rate. Less substrate = higher product.