Study the timeline. A timeline. 4.5 billion years ago, Earth is formed. 4.3 billion years ago, Earliest water. 4.1 billion years ago, Single celled life. 3.5 billion years ago, Earliest oxygen and photosynthesis. 2.5 billion years ago, Complex single celled life. 1.5 billion years ago, Multi cellular life. 500 million years ago, Land life. 300 million years ago, Dinosaurs. 200 million years ago, Mammals. 130 million years ago, Flowers. 5 million years ago, Humans. Based on fossil evidence, about how long ago did the first single-celled life form appear on Earth? 130 million years ago 1.5 billion years ago 2.5 billion years ago 4.1 billion years ago