The number and location of transmembrane segments in an integral membrane protein can be inferred from the amino acid sequence of the protein by a:. a. computer-generated DNA sequence.
b. freeze-fracture replica.
c. prediction of glycosylation sites.
d. Western blot.
e. hydropathy plot.

The number and location of transmembrane segments in an integral membrane protein can be inferred from the amino acid sequence of the protein by a computer-generated hydropathy plot (which is actually a combination of option A and E)

Explanation:

The hydropathi plot is something that enable us to measure the hydrophilicity or hydrophobicity of amino acids in a protein. The computer-generated hydropathy plot identifies the number and location of transmembrane segments. There are plenty of proteins in cells membranes that play different roles and functions. Some of them react to different ph, others with different minerals and other react to water.