You have the following reagents available Rat anti-Keratin 8 antibody specific for mouse Keratin 8, Rabbit polyclonal antibody against mouse keratin 5 and antifade reagent with DAPI to stain DNA. Both primary antibodies work best at 1:500 dilution. A) What additional reagents would you need to perform immunostaining of frozen sections of mouse thymus tissue for Keratin 8 that is expressed in cortical thymic epithelial cells and Keratin 5 that is expressed in mouse medullary thymic epithelial cells? B) Create a staining protocol including all tubes and appropriate controls to stain 4 slides with frozen thymic sections. You will need 200ul of antibody staining solution for each slide.

15 points come and ! in which environment is someone least likely to find fungi growing? a around the sides of a hot tub b on a log in a tropical forest c beneath a mat in a shower d under a rock in the desert

Explain nonnuclear inheritance. ~ ap biology

Cultures of the lake of the ozarks water samples were grown on enrichment media for identification. however, these organisms are present to some degree in all samples. an important question from a clinical perspective is whether or not there is a significant increase in the fecal coliform populations that would put people's health at risk. if you were part of the team investigating this outbreak, how would you best enumerate the fecal coliform threat? view available hint(s) cultures of the lake of the ozarks water samples were grown on enrichment media for identification. however, these organisms are present to some degree in all samples. an important question from a clinical perspective is whether or not there is a significant increase in the fecal coliform populations that would put people's health at risk. if you were part of the team investigating this outbreak, how would you best enumerate the fecal coliform threat? direct microscopic counts filtration count culture serial dilution count culture plate count culture