You decide to perform a chromatin immunoprecipitation to see whether your favorite gene, the Cool gene, is associated with H3 histones that are methylated at lysine 9 (on the histone tail).
You then want to determine whether this methylation also means that expression of the Cool gene is inhibited in cells.
1. Which of the following techniques will allow you to answer this question?
O Northern blot
O DNA footprinting
O Yeast 2 Hybrid
O Quantitative RT-PCR
O Microaray
O Hybridization

Quantitative RT-PCR

Explanation:

Reverse transcription polymerase chain reaction (RT-PCR), also known as quantitative Real-Time (RT) PCR, is a technique widely used in molecular biology laboratories in order to assess the expression level of target genes. This technique employs a transcriptase inverse that enables to obtain DNA from total RNA and then this total DNA is amplified as in a Polymerase Chain Reaction. The levels of the target genes are obtained by comparing their relative expression with one or more control genes.

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