Normal cells die in a nutrient medium containing thymidine and methotrexate, whereas mutant cells defective in thymidylate synthase survive and grow. The mutant cells grow because the medium contains the they are unable to make normal cells, however, continue to synthesize their own and thereby convert their limited supply of to . The methotrexate inhibits , so cannot be regenerated. Without a supply of for the synthesis of nucleotides and amino acids, the cells die.
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