1) The plasmid is 12 KB or 12,000 base pairs (kilo base pairs). If you use a restriction enzyme with one restriction site(cut) how many pieces of DNA do you end up with?
2) Explain the term “double digest”.
3) In the examples of double digests given, are all of the DNA fragments the same size? Why or why not?

Brother ur in collage do it yourself or i will tell prof. mike

The return of the wolf to yellowstone national park will probably affect the bird population to some degree, not significantly. while wolves will hunt birds when necessary, they prefer to hunt larger prey. the bird population will most likely only be affected when hunting is scarce for the wolves. birds of prey will also probably not be affected as much as other birds who spend more time on the ground hunting for food as the tend to spend more time in the air or in trees since they do not need to stay on the ground for long due to the nature of the way they hunt.