G You are performing DNA sequencing reactions as part of your job as a employee at GeneTech, Inc. In your current project, you are attempting to sequence a 700 bp DNA fragment sent by a customer. After the reaction has begun, you realize that due to a decimal error, you accidentally added the fluorescently labeled di-deoxyribonucleoside triphosphates (ddATP, ddGTP, ddCTP, ddTTP) at ten times higher than the desired concentration. What do you expect will be the consequence of this mistake?

The consequence of this error will be the waste of material, since several nucleotides were placed in the reaction and will not be used.

Explanation:

There are several DNA sequencing protocols and although we don't know which one the GeneTech, Inc. employee is using, we can say that the fluorescence-labeled di-deoxyribonucleoside triphosphates will be used to make countless copies of the DNA particle that he wants to sequence.

As we know, DNA replication separates the DNA strands and uses each strand to make copies of new DNA molecules. These copies are made with the DNA polymerase enzyme by adding deoxyribonucleosides to the new tapes. As the enzyme is highly regulated and has a DNA strand as a template for the new strands, it will not add nucleotides beyond what is necessary.

Therefore, by placing excess di-deoxyribonucleoside triphosphates in the reaction, the consequence will be just the waste of product.

americans began to notice growing differences between the northern and southern sections of the country. there were more people living in cities in the north, which made it more urban. more people lived on farms in the south, which made it more rural. while many people were farmers in the northern states, there were now other ways to make a living, such as working in mechanical cotton gins factories manufacturing cloth, tools and other goods. people in the north supported high tariffs on goods imported from europe because the extra cost encouraged people to “buy american” instead. in the southern states, planters did not like tariffs because they sold most of their production to europe and bought their tools from there as well. southerners believed the protective tariff was something that only northern factory owners keep their profits high. so the southern politicians came up with an idea. they would opt out. they would simply ignore the protective tariff. it was ‘null and void’ in south carolina. the state would not collect the taxes on goods imported into south carolina, no matter what the president said they had to do. this decision led to the nullification crisis.