The ability to replace the wildtype alleles with a knockout allele in mice has enabled researchers to carry out important reverse genetics studies on gene function. Please select all of the statements below which correctly describe the creation of gene knockout mice. Multiple answers are possible, and partial credit will be given. A.Illegitimate recombinants have the knockout allele randomly inserted and do not replace the wildtype alleles and may potentially insert into other genes and confound the analysis. B.Resistance to the anti-viral drug ganciclovir indicates that homologous recombination has occured. C.Homologous recombinants of the knockout alleles are desired since they will replace the wildtype allele. D.Resistance to neomycin indicates insertion of the knockout allele but does not distinguish between homologous and non-homologous recombinants. E.The embryonic stem cells carrying homologous recombinants of the knockout allele are placed back into a blastocyst and injected into a surrogate mother to produce mice carrying the knockout allele.

The correct statements are,:

B. Resistance to the anti-viral drug ganciclovir indicates that homologous recombination has occurred.

C. Homologous recombinants of the knockout alleles are desired since they will replace the wildtype allele

E. The embryonic stem cells carrying homologous recombinants of the knockout allele are placed back into a blastocyst and injected into a surrogate mother to produce mice carrying the knockout allele.

Explanation:

wrong statements

A. Illegitimate recombinants have the knockout allele randomly inserted and do not replace the wildtype alleles and may potentially insert into other genes and confound the analysis

D. Resistance to neomycin indicates insertion of the knockout allele but does not distinguish between homologous and non-homologous recombinants

A and D are wrong statement due to the fact that Cells that were transformed with a vector containing the neomycin gene and the herpes tk+ gene are grown in a solution that contains neomycin and Ganciclovir in order to distinguish  the transformations that occured through homologous recombination. Any DNA insertion that occurred through  random insersion( illegitimate recombinant) will die because the tests positive for both  neomycin resistant gene and herpes tk+ gene whose gene product reacts with Ganciclovir to produce deadly toxins.

picture a world where computers are building made-to-order personalized robotic arms and synthetic organs, drones are circulating above mars! due to the rapid growth of technology, the future has never been more unpredictable, and it presents a huge challenge in education: we are teaching students how to work in a future that we have never been unsure of. it is widely predicted that in eight years’ time, there will be more jobs in the fields of science, mathematics, technology and engineering than in any other discipline. and, as the technology continues to develop, it will soon become clear that addressing social problems will involve more innovative thinking, and analytical and technical knowledge than ever before. undeniably, there are no subjects that encourage innovative and creative thinking, deep thought, problem solving and independence more than the stem subjects. while it is widely commented on that there is a need to reimagine the ways stem subjects are taught in schools by embracing a more interactive and innovative approach to appeal to more students, it should come as no surprise that some of the most reputable educational organizations in the world are already implementing changes to embrace stem. harvard, for example, announced in 2014 that they were increasing their computer science faculty by over 50% and has since introduced computer science as a core subject for all freshmen. the urgency of supporting stem subjects at a young age is becoming more relevant than ever, as adopting a keen interest in the curricula needs to happen early. however, beyond the age issue is one of gender.

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