Using BLAST to query a species-specific database

Now that you have determined that the sequence in Part B is part of the human insulin gene, you can use BLAST to search the human-specific (Human genomic + transcript) database. This database lists genomic sequences separately from annotated mRNA transcripts.

BLAST Search Instructions
1. In the search results page of BLAST, click the Edit and Resubmit link in the upper left.
2. In the Choose Search Set box, select the Human genomic + transcript database. In the pull-down menu directly below it, select Human genomic plus transcript (Human G+T).
3. In the Program Selection box, select Highly similar sequences (megablast).
4. Click the BLAST button near the lower-left corner of the page, and wait for BLAST to complete the search.
A new page displays your search results. Again, the Descriptions section of the page lists similar sequences, or hits, with the best statistical match to your query sequence at the top of the list.

Note that in this case, transcripts (mRNA) and genomic sequences are listed separately. You should see several results but only human (Homo sapiens) sequences. If not, see Hint 1 to determine what went wrong.

Based on these BLAST results, what can you conclude about your query sequence? Select all that apply.

1. The sequence is part of only one insulin mRNA transcript.
2. The sequence is part of three distinct insulin mRNA transcripts.
3. The query coverage data indicate that part of the query sequence is not present in any mRNA transcript for insulin
4. The query coverage data indicate that part of the query sequence is not present in the contig for human chromosomei