You encounter a strain of E. coli that is unable to grow or divide using lactose as a carbon source. You analyze the chromosomal genotype of these bacteria, and determine it to be: lacI- lacP lacO lacZ- lacY lacA You want to introduce an F plasmid into this bacterium in order to that restore normal lac operon function: i. e. all three protein-coding genes will be repressed in the absence of lactose and be induced in the presence of lactose. What is the minimum set of genetic elements that you must include on your plasmid in order to achieve this result

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Astudent sees several ants walking up a wall following the exact same trail that an ant took earlier. she wants to apply the scientific method to determine how the ants detected the trail. which of these steps would come first in her application of the scientific method? perform an experiment by cleaning the scent away from part of the trail. draw a conclusion that the ants follow a scent trail. make a prediction about what the ants will do after she cleans away part of the trail. hypothesize that the ants are following a scent trail that the first ant left.