"You are going to use the enrichment culture" technique to isolate Gram negative, aerobic, N2-fixing bacteria from a sample of soil. You will need to design a defined medium which will allow you to enrich for Gram negative, aerobic, N2-fixing bacteria. Which of the following ingredients would you want to include in your defined medium?
A. vitamins
B. Trace Element Solution
C. air (O2 + N2)
D. MgSO4
E. KCl
F. glucose
G. Penicillin
H. yeast extract
I. Na2HPO4
J. NH4Cl
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