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Biology, 11.04.2020 04:06 jaredhoag1982
When glucose is present, the lac genes are not fully expressed, even in the presence of inducer. This is called catabolite repression.
a.. Why does it make biological sense to have the lactose operon under negative control by Lac repressor?
b. CAP is necessary to turn on several sugar operons (including the arabinose, lactose, maltose, and galactose operons). Cells with mutations in CAP cannot efficiently metabolize any of these sugars. On plates that contain a sugar and tetrazolium (an indicator dye), colonies are white if that sugar is metabolized and red if it is not. This kind of plate is often used to screen for cells that cannot metabolize a particular sugar. How could you use these plates to isolate CAP mutants?
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When glucose is present, the lac genes are not fully expressed, even in the presence of inducer. Thi...
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