There are several reasons why the primase used to make the RNA primer for DNA replication is not suitable for gene transcription.

Which of the statements below is not one of those reasons?

a. Primase initiates RNA synthesis on a single-stranded DNA template.

b. Primase can initiate RNA synthesis without the need for a base-paired primer

c. Primase synthesizes only RNAs of about 5–20 nucleotides in length.

d. The RNA synthesized by primase remains base-paired to the DNA template.

The correct answer is option b.

Explanation:

Primase refers to an enzyme that plays an important role in DNA replication, the primers works as the point of initiation for the synthesis of DNA. The primase is a kind of RNA polymerase as it is an enzyme that give rise to short sequences of RNA known as primers.  

Primase after getting associated with a single-stranded DNA template starts the process of the production of RNA. Primase combines with the DNA helicase and gives rise to a complex composition known as primosome. After binding with DNA helicase, primase gets stimulate and begins the production of the short RNA primer. Primase does not require a base-paired primer to start the process of RNA synthesis, however, it gives rise to sequences of RNA, which are approximately 5 base pairs in length.  

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