Shown below is the start of a coding region within the first exon of a gene. 5'--3' 3'--5' What is the PAM sequence of the bottom strand of DNA?

3'-GGT-5'

Explanation:

Protospacer adjacent motif or PAM is an important part of the CRISPR/Cas 9 gene editing system.CRISPR/Cas 9 is a popular gene editing tool that uses an RNA guided endonuclease that cuts DNA at precise target points. As shown in the picture, the CRISPR/Cas9 system consists of the following parts:Cas9 endonucleaseGuide RNA complementary to the target sequence.PAM sequence - a 3 nucleotide sequence on the guide RNA that helps the Cas9 in recognizing and binding to the target DNA. PAM sequences are located 3-6 nucleotides downstream of the target DNA.Every endonuclease recognizes a different PAM sequence. However, the most commonly used Cas9 from Streptococcus pyogenes recognizes the sequence 5' NGG 3'. The "N" represents any nucleotide.

11. gene12. allele13. crossing over14. linked15. gene16. x17. males, femalesapologies if numbers are out of order.

Reactants are mean lol