The major conclusion of the experiment of C. Anfinsen involving ribonuclease was that:

a. a protein can be refolded correctly only if the disulfide bonds are left intact.
b. a denatured protein loses its enzymatic activity.
c. proteins unfold upon heating.
d. reducing agents are required to unfold proteins.
e. the information dictating how the protein should fold is contained in the amino acid sequence.

Explanation:

C. Anfisen was a biochemist that was an American. He got a noble prize in 1972 in the field of chemistry with some other scientists for the ribonuclease. He published many articles to show the relationship between the structure and functions of the proteins. He also worked on genetic material and find out the conclusion that ribonuclease can refold after the denaturation process and the enzyme activity was preserved. Thus the information is encoded in the sequence of the amino acid that is required for the confirmation of the protein.