Biology, 19.03.2020 22:24 aashna66

A protein was purified to homogeneity. The determination of the mass by gel‑filtration chromatography yields a value of 60 kDa. Chromatography in the presence of urea results in a 30 kDa species. Repeating the chromatography in the presence of both urea and β‑mercaptoethanol, however, results in a single molecular species of 15 kDa. What do the data suggest about the structure of the protein?

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Answer from: larey

In the given case, the homogeneity of the protein is done. By performing, the technique of gel-filtration chromatography, the mass of the protein obtained is 60 kilodalton. When the chromatography is performed in the existence of urea, a protein of mass 30 kilodalton is obtained. The ratio between the two obtained mass is 60/30 = 2, thus, it shows that the protein comprises two subunits and each of them weighs 30 kilodaltons.

When the same technique of chromatography is performed in the existence of beta-mercaptoethanol and urea, a single protein of mass 15 kilodalton is produced. Now, the ratio is 30/15 = 2. Therefore, each subunit of the protein comprises two units of mass 15 kilodalton.

Hence, it shows that the protein is a tetramer of 15 kilodalton subunits and each subunit produces dimers of mass 30 kilodalton and is attached by disulfide bonds.

Answer from: Quest

answer: carbon 14 can only be used to determine the age of the organisms that were once living, and igneous rocks are non living.

explanation:

carbon 14 dating is used to determine the age of the sample by estimating the amount of carbon 14 present in the sample and then comparing it with a reference standard.carbon 14 is a isotope of carbon which is radioactive and unstable. carbon 14 is assimilated by all plants and animals throughout their life from carbon dioxide. however, one the organisms die they stop this assimilation and the carbon 14 content starts to decrease.by knowing how much of carbon 14 is left in an organism after his death, one can estimate the age of the organism. the materials whose age can be estimated by carbon 14 method are the organic compounds. however, some inorganic compounds such as aragonite shell can also be dated.however, if we consider the igneous rocks it is a non living component which never accumulated carbon 14 in it and thus, we cannot use carbon 14 dating method to estimate its age.

Answer from: Quest

Then it is june the conclusion to the second sentence is just restating "if it is june then it is summer"

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A protein was purified to homogeneity. The determination of the mass by gel‑filtration chromatograph...

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