The A260/A280 ratio method estimates protein by measuring the absorbance maximum at 280nm caused by the amino acids, , and . Since also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at and using the following equation:
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Body temperature is tightly regulated in mammals for example when external temperatures drop too much the body of a mammal response by in order to it's core temperature.
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The A260/A280 ratio method estimates protein by measuring the absorbance maximum at 280nm caused by...
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