You’ve diluted 100ul of a bacterial stock with 300ul distilled water, but the sample still needs further dilution Step 2: You then take 100ul of the dilution from step 1 and dilute it further with 200ul of distilled water.

The question is inappropriate which should be as the following:

A.) What is the dilution factor after Step 1? (2 points)

B.) What is the final/total dilution factor at the end of Step 2 from the starting stock?

1. Df- 1/4 r 4 fold or 4 times

2. Final dilution factor- 12 times

Explanation:

A serial dilution refers to the reduction in the concentration of the solute in a solution in which the solution is diluted with the same dilution factor.

In the given question,  

The dilution factor will be calculated as the following:

The amount of concentrated solution divided by the total volume of solution

The volume of water added- 300 ul

1. The total volume of solution will be- 100+ 300 ul= 400 ul

Df= 100/400

= 1/4 r 4 fold or 4 times

2.The volume of solution added- 100 ul  

solution added to- 200ul  

The total volume will be- 300  ul  

Df- 100/300 or 3 times

Therefore the final dilution factor will be- 4 x 3= 12

both male and female gametes are produced by meiosis of the germ cells. therefore, both male and female gametes are haploid. one male and one female gamete unite during the fertilization to form the diploid zygote. in seed bearing plants, pollen grain is the male gametophyte, which produces sperm cells.