Biology, 10.03.2020 06:24 naocarolina6
The TCA cycle in a particular tissue has been inhibited by fluoroacetate. However, it is not the fluoroacetate that is responsible for the inhibition. It gets converted to another molecule that enters the TCA cycle and is transformed into the inhibitor.
a. Propose the inhibitor molecule and mechanistically show the conversion of fluoroacetate to the inhibitor.
b. Indicate at what step fluoroacetate ultimately inhibits the TCA cycle.
c. Explain why you think fluoroacetate acts as an inhibitor of this enzyme? Your answer should include some sort of mechanistic explanation.
d. What difference in the concentration of each TCA cycle metabolite would you expect, compared with a normal uninhibited tissue? List each metabolite and indicate the effect on its concentration.
Answers: 1
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